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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Dev Neurobiol. 2012 Jun 25;72(8):1161–1179. doi: 10.1002/dneu.22006

Fig 5. Deletion of CBD domain increases secreted MTG migration at the synapse.

Fig 5

(A) Wandering 3rd instar NMJ synapses expressing wildtype (wt) MTG::GFP (left) or CBD MTG::GFP (right) driven by neuronal elav-GAL4, colabeled with anti-HRP (blue) and anti-GFP (green). Labeling was done without detergent, so that only extracellular proteins are enhanced. Arrowheads show secreted MTG::GFP puncta at a distance from the presynaptic terminal. (B) Higher magnification images showing increased migration from the presynaptic terminal of extracellular ΔCBD MTG::GFP compared with wildtype MTG::GFP. (C) Quantification of mean maximal distance of MTG::GFP puncta from the nearest neuronal membrane. Significance indicated (**) is P=0.003 using Wilcoxon rank sum test and Kruskal-Wallis test.