Table 1. HXT1 expression in strains expressing chimeric sensors.
| Mean β-gal activity, U ± SD
|
||
|---|---|---|
| Chimera construct | 2% galactose | 4% glucose |
| Low-level expression (single copy, RGT2 promoter) | ||
| 1 No chimera (pRS316) | 64 ± 7 | 162 ± 2 |
| 2 Rgt2 (pBM3272) | 70 ± 10 | 6,830 ± 46 |
| 3 Hxt1-tail (pBM4533) | 102 ± 22 | 159 ± 2 |
| 4 Rgt2V404I (pBM4526) | 69 ± 10 | 92 ± 9 |
| 5 Rgt2Δtail (pBM4534) | 63 ± 0 | 137 ± 8 |
| High-level expression (multicopy, ADH1 promoter) | ||
| 6 No chimera (pRS426) | 63 ± 3 | 180 ± 33 |
| 7 Hxt1 (pBM4527) | 73 ± 8 | 226 ± 76 |
| 8 Hxt1-tail (pBM4529) | 897 ± 181 | 2,530 ± 558 |
| 9 Hxt1F371I tail (pBM4530) | 428 ± 13 | 4,150 ± 166 |
| 10 Rgt2 (pBM4528) | 4,320 ± 15 | 6,950 ± 687 |
| 11 Rgt2Δtail (pBM4531) | 238 ± 13 | 4,220 ± 161 |
| 12 Rgt2V404IΔtail (pBM4532) | 187 ± 49 | 401 ± 92 |
| 13 GFP-tail (pBM4524) | 62 ± 1 | 127 ± 4 |
| 14 GFP-tail-CC (pBM4525) | 428 ± 74 | 2,984 ± 356 |
| 15 Yck1-tail (pBM4538) | 422 ± 50 | 1,712 ± 34 |
| 16 Yck1D211A tail (pBM4539) | 80 ± 5 | 123 ± 23 |
| 17 Yck1 cyt (pBM4536) | 25 ± 2 | 93 ± 7 |
HXTI-lacZ is expressed from pBM4523 in YM6107 (Δrgt2 Δsnf3).