Table 2. Split-ubiquitin system interaction between RGT2 and YCK1.
| Mean β-gal acitivity, U ± SD (fold activation over vector alone)
|
||||
|---|---|---|---|---|
| Rgt2-Cub-PLV (pBM4546)
|
Hxt1-Cub-PLV (pBM4547)
|
|||
| NubG plasmid | 2% galactose | 4% glucose | 2% galactose | 4% glucose |
| pNubG-HA-X (vector) | 709 ± 37 | 31 ± 1 | 274 ± 10 | 29 ± 2 |
| NubG-Yck1 (pBM4552) | 807 ± 27 (1.1 X) | 31 ± 5 (1.0 X) | 144 ± 23 (0.5 X) | 26 ± 1 (0.9 X) |
| NubG-Yck1K98R (pBM4553) | 3,435 ± 227 (4.8 X) | 88 ± 14 (2.8 X) | 227 ± 20 (0.8 X) | 31 ± 5 (1.1 X) |
Because the expression of Cub-PLV is higher in cells grown on galactose than in those grown on glucose, we calculated the degree of interaction as the difference in expression of strain between strains carrying Cub-PLV fusions and the empty vector (pNubG-HA-X).