Table 3. Split-ubiquitin system interaction between RGT2 and MTH1 or STD1.
| Mean β-gal activity, U ± SD (fold activation over vector alone)
|
||||
|---|---|---|---|---|
| Rgt2-Cub-PLV (pBM4546)
|
Rgt2Δbox-Cub-PLV (pBM4548)
|
|||
| NubG plasmid | 2% galactose | 4% glucose | 2% galactose | 4% glucose |
| pNubG-HA-X (vector) | 663 ± 76 | 140 ± 16 | 509 ± 134 | 217 ± 7 |
| NubG-Mth1 (pBM4549) | 17,300 ± 568 (26 X) | 291 ± 11 (2.1 X) | 402 ± 30 (0.8 X) | 217 ± 45 (1.0 X) |
| NubG-Mth18ST (pBM4563) | 14,700 ± 452 (22 X) | 1,818 ± 35 (13 X) | 536 ± 28 (1.0 X) | 200 ± 15 (0.9 X) |
| NubG-Std1 (pBM4550) | 2,750 ± 299 (4.1 X) | 202 ± 4 (1.4 X) | 330 ± 58 (0.7 X) | 183 ± 5 (0.8 X) |
| NubG-Std19SA (pBM4551) | 8,650 ± 2030 (13 X) | 883 ± 196 (6.3 X) | 343 ± 6 (0.7 X) | 185 ± 43 (0.9 X) |