Fig. 5.

Unidirectional Ca²⁺/calmodulin-dependent transfer of the lipid-binding domain of VAMP requires fluid-phase trans bilayers. Wild-type VAMP was reconstituted into liposomes with the indicated cis lipid composition. Binding was assayed as in Fig. 3A using trans ³H-liposomes in fluid phase (25% DOPS/75% POPC, gray bars), gel phase (25% DOPS/75% DPPC, cross-hatched bars), or fluid phase lacking acidic headgroups (100% POPC, white bars). Results are the means ± SD of three independent experiments, each in triplicate.