Fig. 1.

EB1-GFP labels new MT ends as they emerge from the centrosome. (A) Time series of EB1-GFP comets emerging from a G₁ centrosome. Each colored arrow follows a single EB1-GFP comet. Typically, each comet was visible for three to four frames. Images were collected by spinning disk confocal microscopy. (B) An interphase LLCPK cell stably expressing EB1-GFP was injected with rhodamine-tubulin and fixed <5 min later. Shown is a single LSM optical section. Both rhodamine tubulin and EB1-GFP brightly label each of the two closely spaced centrosomes in this cell. (Scale bar, 5 μm.)