Fig. 2.

Representative images of EB1-GFP during different stages of the cell cycle. Digital image series from each cell cycle stage were used to count nucleation events per minute. For the telophase cell, only the left centrosome is in focus. In a subset of cells, extra nucleation sites were present (marked by circles). The image labeled Extra Sites was adjusted to show these dim extra nucleation sites. Digital movies of MT nucleation during G₁/S and metaphase are available as Movies 1 and 2. (Scale bar, 10 μm.)