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. 2012 Jul 11;32(28):9639–9648. doi: 10.1523/JNEUROSCI.1064-12.2012

Figure 6.

Figure 6.

Lack of progenitors for hair cells in mesenchymal tissue. A, Separation of sensory epithelium from mesenchymal tissue after treatment with thermolysin is depicted. B, Spheres from sensory epithelium stained for neural stem cell markers and spheres from mesenchyme did not (50 spheres counted; n = 3; asterisk indicates p < 0.05). C, Neurospheres produced from sensory epithelial spheres after separation with thermolysin, expressing nestin, prosensory markers, Sox2, and Lgr5, but not mesenchymal markers, CD105 and Sca1. D, Mesenchymal spheres (positive for nestin, CD105 and Sca1) did not express Sox2 or Lgr5. E, Cells from sensory epithelium formed neurospheres that were labeled for BrdU and gave rise to myosin VIIa-positive hair cells under differentiating conditions. F, When cultured under differentiating conditions, the mesenchymal spheres were labeled for BrdU but did not give rise to cells expressing hair cell markers (Myosin VIIa). The mesenchymal cells differentiated into collagen II-positive chondrocytes and βIII-tubulin-positive neurons.