Fig. 6.

Start1 expression in the PG cells is reduced in the temperature-sensitive ecdysone-deficient mutant ecd-1. Animals were kept at 20°C, the permissive temperature for ecd-1, up to the late second instar. Then one group was allowed to continue development at 20°C, whereas the other group was transferred to 29°C, the restrictive temperature for ecd-1. RNA in situ hybridization was performed on late third instar larvae. WT strain Kochi-R is shown at 20°C(A) and 29°C(B). At this temperature nuclei also show staining. ecd-1 kept at 20°C(C) and 29°C(D) is shown. In ecd-1 at 29°C ring glands are smaller compared to 20°C as observed (35). Only some of the ring glands in the preparation showed staining, and in those only a few cells are stained.