Table 1. Absolute and relative mean differences in lifetime seed capsule production from pairs of developmentally synchronized plants from homozygous T3 independently transformed lines of a WT genotype of N. attenuata, which had been transformed with constructs containing a pi gene fragment in an antisense orientation (AS--, AS-), or an empty vector construct (C1, C2); untransformed plants of the Arizona (A) genotype and plants of the Arizona genotype transformed with constructs containing the full-length pi gene in a sense (S++) orientation.
| Competitors | Mean difference in capsule no. | P | % mean difference in capsule no. | P |
|---|---|---|---|---|
| AS-- vs. C1 | 16.8 ± 2.9 | 0.0003 | 33.2 ± 4.3 | 0.0001 |
| AS--* vs. C1* | 20.8 ± 0.9 | <0.0001 | 54.2 ± 1.8 | <0.0001 |
| AS1-- vs. C1* | 38.4 ± 2.5 | <0.0001 | 64.9 ± 4.5 | <0.0001 |
| AS- vs. C2 | 10.6 ± 1.8 | 0.0032 | 25.7 ± 3.0 | 0.0063 |
| AS-* vs. C2* | 14.0 ± 1.0 | <0.0001 | 36.8 ± 2.8 | <0.0001 |
| AS- vs. C2* | 19.0 ± 3.2 | 0.0005 | 43.3 ± 5.4 | <0.0001 |
| A vs. S++ | 70.2 ± 10.7 | 0.0001 | 53.4 ± 5.3 | <0.0001 |
| A* vs. S++* | 60.3 ± 3.8 | <0.0001 | 60.7 ± 3.5 | <0.0001 |
| A vs. S++* | 136 ± 5.5 | <0.0001 | 75.5 ± 3.2 | <0.0001 |
Two plants were grown in the same pot in three different pair combinations (AS-- vs C1, AS- vs C2, and A vs S++). Plants were either treated with 150 μg of MeJA in 20 μl of lanolin paste (*) to elicit JA-induced defenses or treated with 20 μl of pure lanolin paste as controls; n = 10 per treatment and pair combination. P values are from paired t test comparisons; relative values were arsine square root transformed before analysis.