Dot blots comparing levels of Cyp6g1 transcript in adult flies by using the control Gapdh1 (see Materials and Methods). (a) The first three D. simulans dots are from the Californian lines CS1, CS2, and CS3, whereas the last three are from the African lines AS1, AS2, and AS3. (b) The first two D. melanogaster dots and the fourth dot are from the Californian lines CM1, CM2, and CM3, whereas the third dot and the fifth and sixth dots are from the African lines AM3, AM2, and AM5.