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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Plasmid. 2012 May 8;68(2):93–104. doi: 10.1016/j.plasmid.2012.04.003

Fig. 3.

Fig. 3

The effect of incC2 mutant gene expression on R995ΔincC stability. DF4063 strains containing the plasmids listed below were grown to saturation in media with selection for all resident plasmids. At T=0, the cultures were diluted 105-fold into media with selection only for the incC2-expressing plasmid and grown to stationary phase, at which point the cultures were diluted again. The procedure was repeated until the strains had grown approximately 70 generations. Colonies were screened for the presence of the target plasmid (R995ΔincC) on medium containing kanamycin. All strains contain pR9401 (R995ΔincC). In addition they contain pJAK16 (vector), pRK22429 (tacp-incC2-T7), pRK22457 (tacp-incC2 T16A-T7), pRK22455 (tacp-incC2 K10A-T7) or pRK22456 (tacp-incC2 K15A-T7). Circles: no IPTG; triangles: 0.01 mM IPTG; diamonds: 0.1 mM IPTG; squares: 1 mM IPTG. Error bars refer to the standard error of the mean (SEM). For the sake of clarity, error bars that are less than ±2% are not shown. For each point, the sample size is three (n=3), except for a few points along the curves where the cultures were sampled twice.