Table 1.

Plasmids

Plasmid	Markers	Relevant Genotype	Description	Reference or source
pDB6	Km^r	-	P15A replicon; vector control for pRK2300	Bechhofer & Figurski^a
pJAK16	Cm^r	lacI^q tacp	IncQ replicon; expression vector	Kornacki^b
pR9401	Km^r Tc^r	Δ incC	R995 with 465-bp in-frame deletion within incC	(Siddique and Figurski, 2002)
pR9410	Km^r Ap^r Tc^s	ΔincC P1parABS⁺	pR9401 with P1 parABS locus and an Ap^r marker inserted into the aphA (Km^r) gene, and an EZ::TN <KAN-2> transposon inserted in the tetA-tetR locus; parAB reads in the same direction as aphA	This study
pRK2300	Km^r	korA⁺ ΔincC korB⁺ korF⁺ O_B1⁻	P15A replicon; carries RK2 korA and korB genes; has 465-bp in-frame deletion within incC	(Bechhofer et al., 1986)
pRK22324	Ap^r	korA⁺ ΔincC korB⁺ O_B1⁺	P15A replicon; carries region of RK2 from korAp to korB with 465-bp in-frame deletion within incC (Fig. 2)	(Rosche et al., 2000)
pRK22330	Ap^r	korA⁺ ΔincC korB⁺ korF⁺ korG⁺ kfrA⁺ upf54.8⁺ O_B1⁺ O_B2⁺ O_B3⁺	P15A replicon; carries region of RK2 from korAp to upf54.8 with 465-bp in-frame deletion within incC (Fig. 2)	(Rosche et al., 2000)
pRK22429	Cm^r	lacI^q ϕ[tacp-incC2- T7•Tag]	pJAK16 with incC2-T7 coding region expressed from tacp	This study
pRK22455	Cm^r	lacI^q ϕ[tacp-incC2 K10A- T7•Tag]	pJAK16 with incC2 K10A-T7 coding region expressed from tacp	This study
pRK22456	Cm^r	lacI^q ϕ[tacp-incC2 K15A- T7•Tag]	pJAK16 with incC2 K15A-T7 coding region expressed from tacp	This study
pRK22457	Cm^r	lacI^q ϕ[tacp-incC2 T16A- T7•Tag]	pJAK16 with incC2 T16A-T7 coding region expressed from tacp	This study
R995	Km^r Tc^r	incC ⁺	Natural IncPα plasmid (Fig. 2)	(Villarroel et al., 1983)

D. H. Bechhofer and D. H. Figurski, unpublished results

J. A. Kornacki, unpublished results