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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Plasmid. 2012 May 8;68(2):93–104. doi: 10.1016/j.plasmid.2012.04.003

Table 2.

Walker-like A box mutants of IncC2 are defective for toxicity

Plasmid 1a Relevant properties Plasmid 2a Relevant properties Relative EOP on
double
selectionb
pDB6 vector pJAK16 vector 1.1
pRK2300 korA+ korB+ korF+ pJAK16 vector 0.8
pDB6 vector pRK22429 tacp-incC2-T7 1.1
pRK2300 korA+ korB+ korF+ pRK22429 tacp-incC2-T7 1.3×10−4
pDB6 vector pRK22455 tacp-incC2 K10A-T7 0.8
pRK2300 korA+ korB+ korF+ pRK22455 tacp-incC2 K10A-T7 0.5c
pDB6 vector pRK22456 tacp-incC2 K15A-T7 1.1
pRK2300 korA+ korB+ korF+ pRK22456 tacp-incC2 K15A-T7 1.1
pDB6 vector pRK22457 tacp-incC2 T16A-T7 1.0
pRK2300 korA+ korB+ korF+ pRK22457 tacp-incC2 T16A-T7 1.3
a

EKA13 is the host strain

b

Relative EOP (efficiency of plating) = CFU[+IPTG]/CFU[−IPTG]

c

colonies on IPTG-containing plates are significantly smaller than those on plates lacking IPTG