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Gel retardation assay of various cationic polymers complexed with plasmid DNA. (A) OEI800-SSx, (B) OEI800-SeSex, (C) OEI800-Sex, (D) OEI800, and (E) PEI25k.
Notes: Polymers and plasmid DNA were mixed at various mass ratios and incubated at 37°C for 30 minutes with or without DTT. The mixed suspension was subsequently applied to the agarose gel and electrophoresed at 85 V for 40 minutes.
Abbreviations: DTT, 1,4-dithiothreitol; OEI, oligoethylenimine; PEI, polyethylenimine.
