Fig. 3.

Functional analysis of mutations (-277T → A, -273A → G, and -98A → G) and the -84G/A SNP of the human nNOS exon 1c promoter in TGW-nu-I cells. The indicated DNA fragments containing the wild-type or mutant sequences of the nNOS exon 1c 5′-flanking region were ligated into promoterless/enhancerless firefly luciferase expression vector pGL3-basic. The different reporter gene constructs were transiently cotransfected with the herpes simplex virus thymidine kinase promoter-driven Renilla luciferase expression vector phRL-TK as internal control. pGL3-basic was used as a negative control. Data are expressed as means ± SD of three independent experiments in triplicate.