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. 2012 Apr 19;43(1):31. doi: 10.1186/1297-9716-43-31

Table 2.

Immunoglobulin titres (IgG plus IgM mean log titers and IgG2a/IgG1 log ratios) in mice, measured at week 4 after immunization with bacterins, either physically mixed just with EDA or bound to EDA (EDAvidin) by streptavidin-biotin (BEDA) interaction

Inoculation group
Log10 IgM + IgG
Log10 IgG2a/IgG1 ratio
  (mean ± SD) a (mean ± SD) a
SE-wt live
3.45 ± 0.17
1.18 ± 0.26
SEΔwaaL live
3.60 ± 0.73
1.21 ± 0.10
SEΔgal live
2.02 ± 0.45
1.05 ± 0.07
B-SEΔwaaL + EDA
2.54 ± 0.38
1.05 ± 0.20
B-SEΔgal + EDA
2.69 ± 0.42
0.85 ± 0.12
BEDA-SEΔwaaL
2.77 ± 0.51
1.45 ± 0.20
BEDA-SEΔgal 3.07 ± 0.51 0.74 ± 0.09

a Serum titre was defined as the reciprocal of the highest serum dilution showing a mean O.D. value equal to or higher than that of the negative control sera (n = 4) obtained from mice inoculated with PBS, plus 3 times the SD. Mean and SD (n = 4) represent the data obtained in triplicate.