Table 3.
Rescue of CTCF-deficient ES cells by exogenously introduced GFP-CTCF(L)
| Species | Construct | Deletion in ES cells** | Partial deletion in ES cells** | No deletion in ES cells** | Functional CTCF substitution |
|---|---|---|---|---|---|
| N.a.* |
GFP-CRE |
0% (0/65) |
28% (18/65) |
72% (47/65) |
No |
| Mouse |
GFPCTCFL- |
0% (0/18) |
50% (9/18) |
50% (9/18) |
No |
| Mouse |
CTCFGFP- CRE |
83% (19/23) |
0% (0/23) |
17% (4/23) |
Yes |
| Mouse |
GFPCTCF- CRE |
95% (40/42) |
2% (1/42) |
2% (1/42) |
Yes |
| Chicken | CTCFYFP- CRE | 90% (37/41) | 2% (1/41) | 7% (3/41) | Yes |
*: Not applicable.
**: Percentage and number of clones (between brackets) in which the conditional CTCF alleles were deleted, partially deleted or not deleted are shown.