Table 4.
Oligos
| Oligos used for RACE PCR | |||
|---|---|---|---|
|
Name |
Forward |
Backward |
|
| RACE |
GGACACTCGTATTTGGGCACATTC |
CACAGGGAGCACTTGAAGGGCTTC |
|
|
Oligos used in real-time PCR for ChIP |
|||
|
Name |
Forward |
Backward |
|
| Gal3st1 |
TCCTGGGTGAGGTCAGGAAG |
GGAACTCCGAGTAGCTTCAATG |
|
| Stra8 |
TCCTAGAGAAGGGGGTGTTACC |
AGCTGACCACCACACGTTTTC |
|
| Prss50 |
AGAGGAGGGTAGGGGTATCGAC |
TCGCCTCAGCTAATTTCTAAGC |
|
| Vps18 |
CTGCTGCAGTTCCTCATGTTG |
GTGTGACAGATGGAGGAGCAC |
|
| Chr10 |
AAGGTTGGTAGCTCTGCTTGGACTGCTCG |
AATGTCACAAGCAAAGAAAAGCACGCAAAT |
|
| Amylase |
AATTCTCCTTGTACGGCTTCGTG |
TAGCAATGATGTGCACAGCTGAA |
|
|
Oligos used in real-time PCR for RNA expression |
|||
|
Name |
Forward |
Backward |
|
| Clgn |
TGTCTTCCTTACTCTTCTCTTCCG |
GAAGCCAGGTGAAGCTGAGGTC |
|
| Ctcf |
CCACCTGCCAAGAAGAGAAGG |
GCACCTGTATTCTGATCTTCGAC |
|
| Tcfl5 |
ACGAGATAGGAGGCGCAGAATC |
GTTGTTGCTTTATCTGTCTCCG |
|
| Gal3st1testis-specific form |
GCTACTCGGAGTTCCGGAAA |
GACTTGCAGGGCTTCTTTGG |
|
| Gal3st1 |
ACTGTATCCCAACATGGCCTTC |
ATATCTCGCCGAGGTTGACAC |
|
| Stra8 |
GGCAGTTTACTCCCAGTCTGATA |
CAACTTATCCAGGCTTTCTTCCT |
|
| Prss50 |
GACAGTTCTCTCTGCACTGTGAC |
CACATTTCTTGCTGTTCAGGATA |
|
| Ctcfl |
GCTCTGGCTGTGCACCTTACG |
CCCACTGTGCCACCATCATC |
|
| Ccna1 |
GAGTATGCAGAGGAGATTCATCG |
TCATGTAGTGAGCCTTGGGTCTG |
|
| lpcat2 |
AGCACCCAGTGAGGAAGAGA |
TTCGTAGGTGTGATCCGTCA |
|
| itfg3 |
ACGAGGTGTCTTCTGCCTGT |
GTTCCCACTAAAGCTGCTGG |
|
| dio2 |
TGCAGATCCTGCCAGTCTTT |
CACACTGGAATTGGGAGCAT |
|
| hgf |
GATGAGTGTGCCAACAGGTG |
GGTCAAATTCATGGCCAAAC |
|
| akr1c18 |
CCAGGCCATTCTAAGCAAGA |
TCAGGGAATTTTCCAAGCTG |
|
|
Oligos used in EMSA |
|||
|
Name |
Forward |
Backward |
|
| Stra8 |
GGATCTGTGCTGTGTGTCCTCCTCGACTCCT |
CCTCTAGGAGTCGCAAGTGACCCACACATGCATGC |
|
| |
AGAGCATGCATGTGTGGGTCACTTGCGACTC |
|
|
| |
CTAGAGGA |
TCTAGGAGTCGAGGAGGACACACAGCACAGATCCT |
|
| Prss50 |
ATCTAGGGGGCGCCACGCAGGCTGGGCACC |
CCACAATGGCGCCCTCCATCGGGCGCCTCATGGT |
|
| |
AGCGCACCATGAGGCGCCCGATGGAGGGCG |
GCGCTGGTGCCCAGCCTGCGTGGCGCCCCCTAGATG |
|
| |
CCATTGTGGA |
|
|
|
Oligos used for generating transgenic mice and cells |
|||
|
Name |
|
||
| Oligo 1 |
5-TCTTTTTCCATCAGGGGTCGTCAC-3 |
||
| Oligo 2 |
5-GAGAAGCGCGATCACATGGTCCTG-3 |
||
| Oligo 3 |
5-GCACCGTTTGCAGGGTCAGGATC-3 |
||
| Oligo 4 |
5-TCCAAATCACAGCGCCACCTACAG-3 |
||
| Oligo 5 |
5-GGTTCTTAGAGATAGGGTTTCTCTG-3 |
||
| Oligo 6 |
5-GGTGTTCTGCTGGTAGTGGTC-3 |
||
| Oligo 7 |
5-CGGCATCAGAGCAGCCGATTG-3 |
||
| Oligo 8 | 5-GTTATGATCTGGGTATCGTCCACTG-3 | ||