Figure 3. Human neutrophils form NETs in response to exogenous oxidants.

(A) Hypochlorite (HOCl) release by activated neutrophils incubated in minimal media with or without Cl⁻ was measured using a colorimetric assay for chlorination of extracellular taurine. Production of HOCl by activated neutrophils requires extracellular Cl⁻. Each condition was done in triplicate. (B,C) NETosis increased in dose-dependent manner in response to exogenous hydrogen peroxide and sodium HOCl, as visualized using immunofluorescence and microscopy (blue: DAPI, green: anti-MPO antibody) (B), and using quantification of released extracellular DNA (C) as assays of NETosis. Neutrophils were plated at 2×10⁶ cells/well.