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. 2012 Aug 13;7(8):e43200. doi: 10.1371/journal.pone.0043200

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© 2012 Proust et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, Jurkat cells were untreated or treated with 25 µM of pervanadate for 30 minutes, lysed and immunoprecipited with an anti-CD3ζ antibody. The immunoprecipitates were resolved by SDS-PAGE and transferred, the membranes were subjected to Far-western blotting with GST alone (panel FW GST) then GST-SAP fusion protein (panel FW GST-SAP), stripped and reprobed with an anti-phosphotyrosine (4G10) antibody (upper panel) and then with an anti-CD3ζ antibody (lower panel). B, Jurkat cells were untreated or treated with 25 µM of pervanadate for 30 minutes, lysed, immunoprecipited for CD3ζ and then immunoblotted for SAP (upper panel). The equal amount of precipitated CD3ζ was assessed by an anti CD3ζ blot (lower panel). These experiments were repeated at least 3 times.