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. 2012 Aug 13;7(8):e43200. doi: 10.1371/journal.pone.0043200

Figure 6. SAP-silencing down-modulates IL-2 and IL-4 gene expression in Jurkat cells.

Figure 6

A and B, The Jurkat parental cell (WT) and two clones (ΔSAP-1 and ΔSAP-2), stably expressing two different SAP specific Sh-RNAs (Sh-A3 and Sh-A5 respectively), were stimulated by CD3/CD28 for 60 min for IL-2 (A) or 30 min for IL-4 (B). Quantitative RT-PCR was performed for IL-2 (A) and IL-4 (B) gene expression. Results were normalized to the ribosomal protein, Rpl38, gene expression. Data shown are representative of two independent experiments. C, Whole cell lysates of the Jurkat parental cell (WT) and the two clones ΔSAP-1 and ΔSAP-2 were resolved by SDS-PAGE and SAP extinction was controlled by an anti-SAP immunoblotting. Equal amount of proteins in each lane were assessed by an actin-immunoblotting. Each band was numerically quantitated (indicated in arbitrary units, a.u.) and the SAP/Actin ratio for each cell population is graphically presented (normalized to 100% in Jurkat WT).