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. 2012 Aug 13;7(8):e42994. doi: 10.1371/journal.pone.0042994

Figure 8. GCAP1 localizes to the synaptic terminal and partially overlaps with Ribeye.

Figure 8

Immunolabeling of vertical retinal sections from WT and GCAPs−/−GCAP2+ mice with rabbit polyclonal antibody anti-GCAP1 and a monoclonal antibody against Ribeye/CtBP2. GCAP1 is found at the outer segment (os) inner segment (is) and outer plexiform layer (opl) of the retina, where it colocalizes with Ribeye at synaptic ribbons (white arrows). GCAP1 antibody immunolabeling signal was absent in GCAPs−/−GCAP2+ sections when identical laser power and acquisition gain parameters were used at the confocal microscope, excluding that the signal originates from cross-reactivity of anti-GCAP1 antibody with GCAP2 at this working dilution.