Figure 4. Knockdown of G3BP impairs formation of avSG and IFN-â gene activation.

(A–E) HeLa cells were transfected with control siRNA (N.C) or siRNA targeting human G3BP (G3BPi). At 48 h after transfection, cells were harvested and G3BP and actin were detected by immunoblotting (A). Cells were mock-treated (mock) or infected with IAVÄNS1 for 12 h and fixed and stained with anti-RIG-I, anti-NP and anti-TIAR antibodies and DAPI (B). The percentage of cells containing foci of RIG-I (C) or TIAR (D) was determined. Relative mRNA level of IFN-â was determined by qPCR (E). Data are represented as the mean standard ± error of the mean (SEM).