Figure 7. Inhibition of avSG formation enhanced IAV viral replication.

HeLa cells were transfected with control siRNA (N.C) or siRNA targeting human PKR mRNA or G3BP. At 48 h after transfection, cells were mock-treated or infected with IAVÄNS1 for 24 h. The expression levels of IAV RNA segment 3 and GAPDH mRNA were determined by RT-PCR (top). The IAV RNA expression patterns were quantified with LAS-1000 UV mini (Fujifilm, Japan) and normalized with GAPDH (bottom). Data are represented as the mean standard ± error of the mean (SEM).