Figure 8. Viral RNA and polyI:C induce formation of avSGs and IFN-â gene expression in a PKR-dependent manner.

(A–C) MEFs derived from WT and PKR KO mice were mock-treated or transfected with IAV genomic RNA, short poly I:C or long poly I:C for 9 h and stained with anti-RIG-I, anti-G3BP antibodies and DAPI (A). The percentage of cells containing foci of G3BP was shown in (B). Relative IFN-â mRNA levels were determined by qPCR (C). Data shown in B and C are represented as the mean standard ± error of the mean (SEM).