Skip to main content
. 2012 Aug 13;7(8):e43041. doi: 10.1371/journal.pone.0043041

Figure 6. PTRF/cavin-1 expression in PC3 cells impairs Rac1 polarization, but leads to recruitment of PKCα to detergent-resistant membrane (DRM) fraction.

Figure 6

(A) Rac1 polarization was examined by confocal immunofluorescence microscopy. Polarization was measured by fluorescence intensity along the length of the cell as indicated by red lines shown. For quantitation, 20 cells over 3 independent experiments were analysed, and Rac1 was deemed polarized when staining was confined to terminal one third of the cell body. P<0.0001. (B) PTRF/cavin-1-GFP expression in PC-3 cells caused recruitment of PKCα to the lipid raft (DRM) fraction, without altering total cellular level or total membrane (P100) level. (C) Loss of PKCα from lipid raft (DRM) fraction in NIH3T3 fibroblasts with PTRF/cavin-1 knockdown.