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(a) RT/PCR amplicon of CTNS (spanning exons 10 to 12) obtained from RNA extracted from amMSC microvesicles. (b) RT/PCR showing absence of CTNS mRNA in GFP-tagged CTNS(−/−) mutant fibroblasts isolated by FACS after 4 days of co-culture with untagged mutant fibroblasts (lane 1); amplicons are evident in FACS-isolated GFP-tagged mutant fibroblasts after 4-day co-culture with amMSC (lane 2) and in control amMSC (lane 3).
