Figure 5. Toxoplasma gondii infection does not lead to wasting or lymphopenia in Foxp4 cKO mice.

Eight-week-old Foxp4^FLOX/FLOXCre^NEG, cHET and cKO mice were infected with T. gondii by intraperitoneal injection of 20 Me49 cysts. A) Weights of mice were assessed longitudinally and plotted relative to the percentage of each respective mouse's starting weight at d0. Mean and s.d. from 9 Cre^NEG, 12 cHET, and 15 cKO mice. B) Brain tissue was harvested at day 40 post infection and parasite DNA quantitated using SYBR probes specific for T. gondii. Levels of T. gondii DNA were normalized to a standard curve. Each point represents an individual mouse. Mean and standard deviation are shown. Representative of 3 individual experiments. C) Spleens were harvested at 40 days post infection and total spleen cellularity was determined. Each point represents and individual mouse. Representative of 3 experiments.