Table 1. Effects of exemestane on Annexin V-PE labelling in MCF-7aro cells.
6 DAYS | T | T+5 µM | T+10 µM | T+15 µM | T+STS 1 µM |
AnnexinV−/7-AAD− | 86.69±1.62 | 84.45±0.15 | 74.47±2.53* | 73.24±3.44* | 59.87±1.08*** |
Annexin V+/7-AAD− | 3.67±0.35 | 5.62±0.45 (1.53) | 9.80±0.92** (2.67) | 10.04±0.61*** (2.74) | 17.40±1.15*** (4.74) |
Annexin V+/7-AAD+ | 9.65±1.28 | 9.94±0.30 (1.03) | 15.57±1.65 (1.61) | 16.73±2.85 (1.73) | 20.38±2.10** (2.11) |
Cells were treated with different concentrations of exemestane for 6 and 9 days. Treated cells were harvested and labeled with Annexin V-PE and 7-AAD followed by flow cytometry analysis. Data are presented as viable cells (Annexin V-/7-AAD-), early apoptotic (Annexin V+/7-AAD-) and late apoptotic or necrotic cells (Annexin V+/7-AAD+). Cells cultured with T at 1 nM were considered as control and cells treated with T plus STS (1 µM) were considered as positive control for apoptosis. The data represents means ± SEM of three independent experiments done in triplicate. The ratio treatment/control is presented in bold within brackets. Significant differences between the control versus treated cells are indicated by
(p<0.05),
(p<0.01) and
(p<0.001).