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. 2012 Aug 13;7(8):e42718. doi: 10.1371/journal.pone.0042718

Figure 4. Effects of pH/pCO2 on shell mineralogy in the juvenile tubeworm, Hydroides elegans.

Figure 4

(a) The infrared absorption spectrum obtained from shell samples of the control pH/pCO2 (pH 8.1) using the Fourier transform infrared spectrometry (FT-IR). (b) Effects of pH/pCO2 on the magnified portion, between wavelengths 600–900 cm−1, of infrared spectral pattern of the shell. (c) Effect of pH/pCO2 on the intensity ratio (Imax) of the two absorption peaks at 855 cm−1 (ν 2) and 713 cm−1 (ν 4). The Imax ν 2/Imax ν 4 ratio is an indicator of amorphous calcium carbonate (ACC) content. Each bar represents the mean ± SD of 5 biologically independent measurements. The asterisk (*) shown on the bar indicates a significant difference between the control and the treatment according to the Dunnett's Post-hoc test (p<0.05).