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. 2012 Aug 13;7(8):e42823. doi: 10.1371/journal.pone.0042823

Table 4. Specificity of antibodies directed against mouse GFAP isoforms tested on SW13/cl.2 cells transfected with mouse full length GFAP isoform cDNAs.

Transfection GFAPpan GFAPmono GFAPc-term msGFAPδ msGFAPκ msGFAP+1
Rabbit Mouse Goat Rabbit Rabbit Rabbit
1∶6,000 1∶3,000 1∶3,000 1∶3,000 1∶3,000 1∶6,000
GFAP-α + + +
GFAP-δ* + + +
GFAP-κ + + +
GFAP-Δ135 + + **
GFAP-Δ164 + +
GFAP-ΔEx6 + +
GFAP-ΔEx7 + +
*

Transfection with human GFAPδ showed panGFAP positive cells that were negative for msGFAPδ.

**

GFAPd135 is expected to have a conventional GFAPα C-terminus and the observed absence of staining.