Table 4. Specificity of antibodies directed against mouse GFAP isoforms tested on SW13/cl.2 cells transfected with mouse full length GFAP isoform cDNAs.
Transfection | GFAPpan | GFAPmono | GFAPc-term | msGFAPδ | msGFAPκ | msGFAP+1 |
Rabbit | Mouse | Goat | Rabbit | Rabbit | Rabbit | |
1∶6,000 | 1∶3,000 | 1∶3,000 | 1∶3,000 | 1∶3,000 | 1∶6,000 | |
GFAP-α | + | + | + | − | − | − |
GFAP-δ* | + | + | − | + | − | − |
GFAP-κ | + | + | − | − | + | − |
GFAP-Δ135 | + | + | −** | − | − | − |
GFAP-Δ164 | + | − | − | − | − | + |
GFAP-ΔEx6 | + | − | − | − | − | + |
GFAP-ΔEx7 | + | − | − | − | − | + |
Transfection with human GFAPδ showed panGFAP positive cells that were negative for msGFAPδ.
GFAPd135 is expected to have a conventional GFAPα C-terminus and the observed absence of staining.