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. 2012 Aug 15;23(16):3041–3056. doi: 10.1091/mbc.E12-03-0194

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© 2012 Malinovska et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Sis1 localizes to stress-inducible compartments that contain misfolded proteins and molecular chaperones. (A) Fluorescence microscopy of BY4741 yeast cells expressing Sis1-GFP and mCherry-VHL or GFP-Ubc9ts and Sis1-mCherry. The cells were incubated at 37°C for 1 h in the presence of MG132. (B) BY4741 yeast cells carrying a GFP-tagged chromosomal copy of SIS1 and an mCherry-tagged chromosomal copy of HSP104, HSP42, or SSA1 were observed by fluorescence microscopy after growth at 37°C for 1 h.