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. 2012 Aug 15;23(16):3203–3214. doi: 10.1091/mbc.E12-01-0034

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© 2012 Ito et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Three-dimensional deconvolution observations of the Golgi markers and ERES. BY-2 cells were observed by SCLIM with optical slices 0.2 μm apart on the Z-axis. 3D images were reconstructed and deconvolved by parameters optimized for the Yokogawa spinning-disk confocal scanner. (A) GFP-SYP31 (cis, green) and ST-mRFP (trans, magenta). Before BFA treatment (top) and after 2 h of 50 μM BFA treatment (bottom). Arrowheads indicate the punctate compartments of GFP-SYP31 on the tips of the tubular ER. (B) SEC13-YFP (ERES, green) and mRFP-SYP31 (cis, magenta). Before BFA treatment (top) and after 1.5 h of 50 μM BFA treatment (bottom). Scale bars, 2 μm.