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Levels of circulating Igs and autoantibodies in 1-year-old ERα-/- mice. Level of circulating Igs in 1-year-old mice was assayed by ELISA. (A) ODs at 450 nM were compared between ERα-/- and WT mice (four mice per group). (B) Serum titers of anti-dsDNA IgG in 1-year-old mice were analyzed by using an ELISA kit, and OD values of a step-wise dilution factor from one set of representative results are shown. (C) Summary of preference of anti-dsDNA IgG in 10 ERα-/- mice is shown.
