Table 2.
mRNA expression profiles of stem cell mediators in colonic mucosa from control and DSS treated mice - 3 days of recovery.
| Gene | Diet | Expression Level (% of control) | pvalue vs control | pvalue CO vs FO |
|---|---|---|---|---|
| Ascl2 | CO | 61.3 | <0.001 | |
| FO | 24.1 | 0.002 | 0.016 | |
| Axin2 | CO | 53.1 | <0.001 | |
| FO | 28.8 | 0.002 | 0.016 | |
| EphB3 | CO | 65.8 | <0.001 | |
| FO | 43.7 | 0.002 | 0.032 | |
| STAT3 | CO | 168.8 | <0.001 | |
| FO | 161.3 | <0.001 | 0.111 | |
| Hopx | CO | 70.0 | 0.006 | |
| FO | 64.7 | 0.002 | 0.191 | |
| Lgr5 | CO | 43.8 | 0.008 | |
| FO | 24.1 | 0.002 | 0.191 | |
| Sox9 | CO | 59.9 | <0.001 | |
| FO | 50.8 | <0.001 | 0.310 | |
| Rspo-1 | CO | 114.6 | 0.550 | |
| FO | 128.0 | 0.413 | 0 342 | |
| mTERT | CO | 86.1 | 0.243 | |
| FO | 71.6 | 0.122 | 0 421 | |
| Bmi1 | CO | 127.3 | 0.222 | 0.548 |
| FO | 113.2 | 0.030 |
Real-time PCR was performed using Taqman probes (Applied Biosystems) and expression levels were normalized to ribosomal 18S. Values are means, n=5 mice per group. TaqMan gene expression assay IDs were as follows: Ascl2, Mm01268891_g1; Axin2, Mm00443610_m1; Bmi1, Mm03053308_g1; EphB3, Mm00802553_m1; Hopx, Mm00558630_m1; Lgr5, Mm00438890_m1; mTert, Mm00436931_m1; Rspo1, Mm00507077_m1; Sox9, Mm00448840_m1; Stat3, Mm01219775_m1; 18S, Hs99999901_s1.