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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Mol Microbiol. 2012 Jul 11;85(4):782–794. doi: 10.1111/j.1365-2958.2012.08139.x

Figure 7. Detecting the interactions between two CheAs and three CheWs of B. burgdorferi by co-IP.

Figure 7

Pull down of the CheWs using αCheA1 (right panel) or αCheA2 (left panel). Precipitated proteins were probed with αCheW1 (A), αCheW2 (B), or αCheW3 (C). A previously constructed cheA1A2 double-deletion mutant (ΔA1A2) of B. burgdorferi (Li et al., 2002) was used as a negative control for the co-IP. (D) Pull down of CheA2 using αCheW1 (left panel) and αCheW3 (right panel). Precipitated proteins were probed with CheA2. Extracts from the ΔW1 or ΔW3 mutants were used as negative controls for the co-IP.