A. Representation of the strategy followed to fuse mCherry to A. nidulans HhoA, and GFP to RtfA respectively. The tagged constructs were introduced at the hhoA and rtfA loci, respectively, by double-recombination events.
B. Micrographs showing the subcellular localization of the RtfA::GFP fusion described in (A) in A. nidulans. From left to right, Normaski images, red fluorescence images, and green fluorescence images are included. Red fluorescence images correspond to HhoA::RFP fusion used as nuclear localization internal control.