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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Mol Microbiol. 2012 Jul 12;85(4):795–814. doi: 10.1111/j.1365-2958.2012.08142.x

Fig. 2. RM3 presents a mutation in the rtfA gene.

Fig. 2

A. Complementation insert from the genomic library plasmid in the RM3 mutant and corresponding genomic fragment in the RM3 mutant. Arrow indicates the point mutation at rtfA in RM3, a G-T transversion at nucleotide 915 of the rtfA ORF (from GGA to TGA resulting in a non-sense mutation. coq7 is an adjacent putative ubiquinone biosynthetic gene.

B. Alignment of deduced amino acid sequences of A. nidulans RtfA and putative orthologs in A. oryzae, A. flavus, A. terreus, A. clavatus and S. cerevisiae. The alignment was performed using ClustalW (http://www.ebi.ac.uk/Tools/clustalw2/index.htm) multiple sequence alignment software program. Conserved residues are highlighted in black (identical) and grey (similar). Black and gray underlining indicates two conserved domains present in S. cerevisiae Rtf1 (amino acid residues, 62 – 152 and 201 – 395), demonstrated to be involved in histone methylation, telomeric silencing (black and grey) and ORF association (gray).