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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Mol Microbiol. 2012 Jul 12;85(4):795–814. doi: 10.1111/j.1365-2958.2012.08142.x

Fig. 5. Effect of the rftA deletion on the expression of aflR and stcU over time.

Fig. 5

A. Wild type (WT) veA+ control (TRV50), ΔrtfA (TRVΔrftA) and complementation strain (TRVΔrftA-com) were inoculated in liquid GMM. Mycelia were collected after 24, 48 and 72 hrs of culture in a shaker incubator at 250 rpm at 37°C. Expression of aflR and stcU was analyzed by Northern blot. rRNA serves as loading control. The densitometry was carried out with the Scion Image Beta 4.03 software. Asterisk indicates not detected. ST band intensity values were normalized with respect to the highest intensity considered as 1.

B. TLC showing accumulation of ST in the cultures described in (A).