Table 2.
PCR primers used in the study
| Name | Sequence (5’ → 3’) |
|---|---|
| TF1 | TCAACTCGTCGTCCAGCTCTTCTCC |
| TF2 | AGTGAATGAATTTGCTGACAGCCGACG |
| TF3 | GTGACTGAGACGGTGCAGCATC |
| TF4 | TTCCGTGGTAATGTTGAGTAGACGC |
| Afp1 | CGTCGGCTGTCAGCAAATTCATTCACTGCCTCAAACAATGCTCTTCACCC |
| Afp2 | GATGCTGCACCGTCTCAGTCACCTGTCTGAGAGGAGGCACTGATGC |
| RM3-f1 | CACTACATGAACGTGTTGGTGGCG |
| RM3-r2 | TCACGTACTTTGTCGCCTTAGGGC |
| TF5 | AAGGTTGGCGAGGCTTCAGAGG |
| TF6 | TCTCCCAACTTCATCGTCATCACCG |
| pyrGAngsp1 | CAGCCATCCCACTTCCAGCTTC |
| pyrGAngsp2 | CTGGTAATACTATGCTGGCTGC |
| RtfAcom1 | AAAAAACCGCGGTCGCAAGCATATCCTTCAACTCG |
| RtfAcom2 | AAAAAAGGTACCTTAGGCAGTGGGTATGATGTTGG |
| Rtf-OE1 | AAAAAAGGTACCATGGCCGATGATCTCGACG |
| RtfA-OE2 | AAAAAATTAATTAA CAAGTTCTAAATTTCAATATCGATGTCC |
| FusionMarkerLinkerR | GTCTGAGAGGAGGCACTGATGCG |
| RM33UTRFusionF | CTTGAATATATTCGGTAAAAGATGCTTTTATTCTGC |
| RM33UtrFusionR | GCCTTCTTCTCCGCTTCAGCC |
| RM35UTRFusionR | AGCATACAAGAGACAGCGAGAGC |
| RM3GFPFusionF | CCTGGATCTGGACATCGATATTGAAATTGGAGCTGGTGCAGGCGCTGGAGCC |
| RM3GFPFusionR | GCAGAATAAAAGCATCTTTTACCGAATATATTCAAGGTCTGAGAGGAGGCACTGATGCG |
| actin-F | ATGGAAGAGGAAGTTGCTGCTCTCGTTATCGACAATGGTTC |
| actin-R | CAATGGAGGGGAAGACGGCACGGG |
| nsdD-F | CATCTCACCAGCCACAATTACAGGCGGAACCATCAC |
| nsdD-R | TTGCGAGCCAGACACAGAGGTCATAACAGTGCTTGC |
| steA-F | TCCAGCAAATGGAACCGTGGAATCAGGTGCTC |
| steA-R | GAAGGGATGGGGCAAGAATGAGACTTCTGCGGGTAA |
| brlA-F | AGCTGCCTGGTGACGGTAGTTGTTGTTGGTGTTGC |
| brlA-R | CAGGAACGAATGCCTATGCCCGACTTTCTCTCTGGA |
| S1-822 | TGACCACGAGCACCCCCATC |
| S2-823 | ACGCATGGTGGCAGGCTTTG |
| S3-824 | TCTGCCGGCGTTTATTTGTCA |
| S4-825 | TCGAGAGGCTGAAGCAGAAGACA |
| S5-826 | CAAAGCCTGCCACCATGCGTGGAGCTGGTGCAGGCGCTGGAG |
| S6-827 | TGACAAATAAACGCCGGCAGACTGTCTGAGAGGAGGCACTGAT |
| hhoAF | GCCTCCCAAGAAAGCTCCCACTACGGCC |
| hhoAR | GGCCTTCTTGTTCTTAGCAGTCTTCTTGGTCGCC |
| hhoA_mRFP_F | GGCGACCAAGAAGACTGCTAAGAACAAGAAGGCCGGAGCTGGTGCAGGCGCTGGAGCC |
| hhoA_mRFP_R | CGCTCAAACAATCTCCAAGCCAGGCTGCCTGTTTAGGCGCCGGTGGAGTGGCGGCCC |
| ANhhoA_3UTR_F | ACAGGCAGCCTGGCTTGGAGATTGTTTGAGCG |
| ANhhoA_3UTR_R | AAAAAAGGTACCGGGGGAACAACGGAGCCGGTCCAGTGG |
| rtfADHfor | AAAAAAAGATCTGTATGGCCGATGATCTCGAC |
| rtfADHrev | AAAAAAAGATCTCTAAATTTCAATATCGATGTCCA |