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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2012 May 18;1822(10):1516–1526. doi: 10.1016/j.bbadis.2012.05.007

Figure 5. Impact of p.K161N and p.D175N substitutions on hGALE function in living yeast.

Figure 5

Cultures of gal10-null yeast expressing either WT, p.K161N-hGALE, p.D175N-hGALE, or no hGALE were inoculated into 96-wellplates at a density of 5 × 105cells/ml in SGE-Ura medium with the indicated amount of galactose added at t = 0. Growth of each culture was monitored as described in Materials and Methods. Values represent means ± SD; n ≥ 6. Resulting growth profiles are demonstrated for all four strains cultured in the presence of medium containing (a) 0 %, (b) 0.002%, (c) 0.01 % and (d) 0.02 % galactose.