Table II.
Effect of DMPO on the cysteine sulfonation at the C554 and C727 of the Complex I from the reaction mixture containing Complex I, NADH, and Q1
| Treatment | m/z (Theoretical) | m/z (Observed) | Peptide Sequence and OPTM |
|---|---|---|---|
| −DMPO (Ta) | 765.86822+ | 766.182+ | 544M(ox)LFLLGADGGC554(SO3)ITR557 |
| +DMPO (T) | 757.87072+ | 758.172+ | 544M(ox)LFLLGADGGC554(SO2)ITR557 |
| −DMPO (TCb) | 562.27422+ | 562.372+ | 547LLGADGGC554(SO3)ITR557 |
| +DMPO (TC) | 554.27682+ | 554.682+ | 547LLGADGGC554(SO2)ITR557 |
| −DMPO (T) | 780.84602+ | 780.932+ | 713AVTEGAHAVEEPSIC727(SO3) |
| +DMPO (T) | 772.84852+ | 772.692+ | 713AVTEGAHAVEEPSIC727(SO2) |
a
T: Trypsin digestion of the sample
b
TC: Trypsin+Chymotrypsin digestion of the sample