Table 1. Biochemical and phenotypic differences between wild type (mtB6) and mutant (mtBALB) cybris cells.
mtBALB cybrids with genetic alteration in mt-Tr gene (9821insA) showed significant biochemical changes such as diminished levels of complex I protein, less cellular oxygen consumption, and lower ATP levels. These cybrids exhibited increased levels of ROS, resistance to UV-induced apoptosis and changes in cell growth, migration and invasion capabilities [18, 19].
| mtBALB relative to mtB6 | ||
|---|---|---|
| Biochemical changes | ATP production | ↓ |
| Complex I levels | ↓ | |
| Oxygen consumption (baseline) | ↓ | |
| Oxygen consumption (CII substrates) | ↑ | |
| Levels of mitochondrial ROS | ↑ | |
| Phenotypic changes | Proliferation rate | ↑ |
| Resistance to UV-induced apoptosis | ↑ | |
| Migration | ↑ | |
| Invasion | ↑ | |