Fig. 2.

Immunoprecipitation analysis of HSP70, Bax, Bcl-2 in total cellular proteins isolated from MKN7 cells coincubated with Hp expressing cagA and vacA with addition of exogenous CagA. Amount of 10 μg of total cellular proteins was loaded per each lane. Proteins extracts from control cells (lane 1) and cells coincubated with Hp with addition of exogenous CagA over 3 h (lane 2), 24 h (lane 3) and 48 h (lane 4) were separated in 12 % SDS-polyacrylamide gel. Blots were probed with anti human HSP70, Bax, Bcl-2 antibody, respectively. Mean ± SEM of four determinations. Asterisks indicate a significant (p < 0.05) increase or decrease above the control values. Double asterisks indicate a significant (p < 0.05) increase or decrease above or below the values recorded at 3 and 24 h, respectively