Fig. 4.

RT-PCR analysis of mRNA expression for COX-2 in the MKN7 cells exposed to Hp expressing cagA and vacA and coincubated with the selective COX-2 inhibitor, NS-398. a and b Represent mRNA expression for β-actin and COX-2, respectively. Lane control means control cells without Hp infection and without coincubation with NS-398. Following lanes represent mRNA expression for β-actin and COX-2 in MKN7 cells infected with Hp and incubated with NS-398 for 3, 6 and 24 h, respectively. Line NS-398 means MKN7 cells incubated with NS-398 only. c Represents the ratio of COX-2 mRNA over β-actin mRNA. Results are mean ± SEM of five experiments. Asterisk indicates a significant change (p < 0.05) as compared to control