Fig. 1.
pex6 peroxisome-defective phenotypes. (A) Root elongation on IBA. Seeds were plated on PN plus 15 mM sucrose and the indicated IBA concentrations and grown under yellow filters for 8 days. (B) Lateral root initiation on 15 mM sucrose. Five-day-old seedlings were transferred from hormone-free medium to medium containing no hormone or IBA. Lateral roots were counted after 5 additional days. (C and D) Sucrose dependence during seedling development. (C) Hypocotyl elongation on increasing sucrose concentrations in the dark. Seeds were plated on the indicated medium and incubated under white light overnight, followed by 5 days in the dark. Symbols are as in A.(D) Root elongation on 0 or 30 mM sucrose. Seeds were plated and grown in the dark as in C or in yellow light for 13 days when root lengths were measured. Symbols are as in B. Error bars indicate SEM (n ≥ 9). The peroxisome-defective pxa1 mutant (6) is shown for comparison.