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. 2012 May 25;9:100. doi: 10.1186/1742-2094-9-100

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Copyright ©2012 Min et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Iba-1⁺/CD45⁺cells appear and express phagocytic activity in injured spinal cords. (A, B, E, F) Sections from intact, P1, or P2 regions at the indicated times after the injury were labeled with indicated antibodies. White arrows indicated positively labeled cells with each antibody. (B) Black arrows (P1, 7 d) indicated CD45⁺ cells that are not round. (C, D) Spinal cord tissues were obtained at the indicated times after SCI, and total RNA and protein were prepared and analyzed by RT-PCR (C) and Western blotting (D), respectively, as described in Materials and methods. Data shown are representative of at least three independent experiments. Scale bars: 20 μm.