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. 2012 Jun 12;9:51. doi: 10.1186/1742-4690-9-51

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Copyright ©2012 Liu and Eiden; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The binding affinity of GALV RBD with MDTF cells expressing HA tagged PiT1, PiT2K522E or PiT2. GALV RBD tagged with both V5 and His was purified using high-performance nickel-NTA (Ni-NTA) agarose, serially diluted four times and applied to a binding assay for MDTFPiT1-HA (A) or MDTFPiT2K522E-HA and MDTFPiT2-HA cells (B). The kinetics of binding of GALV RBD was compared. The mean fluorescence intensity (MFI) for GALV RBD binding is indicated on the y-axis and GALVRBD concentration (mg) on the x-axis. Three independent experiments were performed and the mean ± standard deviation was presented in the curve.