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. 2012 Jun 28;9:147. doi: 10.1186/1742-2094-9-147

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Copyright ©2012 Nikodemova and Watters; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis of the CD11b⁺fraction. (A) After isolation, cells in the CD11b⁺ fraction were stained with anti-CD45 antibodies and analyzed by flow cytometry. Two CD45-expressing populations were identified: low (red) and high (blue). (B) Forward scatter (FSC) and side scatter (SSC) characteristics of CD11b⁺/CD45^low (red) and CD11b⁺/CD45 ^high (blue) are shown. A histogram of FSC parameters comparing CD45^low and CD45^high cells is shown on the right. (C) Histogram showing the differences in CD11b expression between CD45^low and CD45^high cell populations.